In the early 1950s, experiments by two teams of researchers, Salvador Luria working with Mary Human and Joe Bertani working with Jean Weigle, showed that some strains of bacteria were more resistant ...
Cold Spring Harbor, NY-- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of Restriction Enzymes: A History, available on its website in hardcover format. Restriction enzymes cleave ...
The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...
Most restriction endonucleases use Mg²⁺ to hydrolyze phosphodiester bonds at specific DNA sites. We show here that Bfil, a metal-independent restriction enzyme from the phospholipase D superfamily, ...
Plastid DNA was isolated from the chloroplasts of tomato (Lycopersicon esculentum var Traveler 76) leaves and the chromoplasts of ripe tomato fruit. Comparisons of the two DNAs were made by ...
Modular cloning and expression of complex genetic assemblies are becoming widely popular. Recent applications based on site-specific and homologous recombination have substantially improved the ...
Key to Invitrogen's Gateway technology is the "entry clone," which contains a fragment of interest in a recombinase-ready plasmid. Researchers can quickly and efficiently move between expression ...
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